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Image Search Results
Journal: bioRxiv
Article Title: Conserved Cx n C motifs in Kaposi’s sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and mediate its interaction with ORF34
doi: 10.1101/728139
Figure Lengend Snippet: A) Diagram of the vPIC components in KSHV. Line weights indicate relative strength of interactions. B) ORF18, ORF31, and ORF66 can immunoprecipitate the entire vPIC complex. HEK293T cells were transiently transfected with FLAG- or Strep-tagged vTAs and a co-IP was performed using anti-FLAG magnetic beads. In (C-E), StrepTactinXT magnetic beads were used to isolate Strep-tagged ORF66 and demonstrate ORF18 (C), ORF31 (D), and ORF34 (E) co-IP with ORF66.
Article Snippet: Plasmids pcDNA4/TO-ORF18-2xStrep (Addgene plasmid # 120372),
Techniques: Transfection, Co-Immunoprecipitation Assay, Magnetic Beads
Journal: bioRxiv
Article Title: Conserved Cx n C motifs in Kaposi’s sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and mediate its interaction with ORF34
doi: 10.1101/728139
Figure Lengend Snippet: A-C) HEK293T cells were transiently transfected with wild-type or mutant Strep-tagged ORF66 and the indicated FLAG-tagged vTA, then co-immunoprecipitated with StrepTactinXT beads followed by Western blotting. D) HEK293T cells were transiently transfected with wild-type or mutant Strep-tagged ORF66 along with FLAG-tagged ORF18, ORF24, ORF30, ORF31, and ORF34. A co-IP with anti-FLAG magnetic beads was performed followed by Western blotting.
Article Snippet: Plasmids pcDNA4/TO-ORF18-2xStrep (Addgene plasmid # 120372),
Techniques: Transfection, Mutagenesis, Immunoprecipitation, Western Blot, Co-Immunoprecipitation Assay, Magnetic Beads